ACSL4 and hepatocellular carcinoma: After stimulation of HepG2 cells with 40 μM curcumin for 24 h, We examined the viability of HCC cells and found that the viability of hepatocellular carcinoma cells was not significantly reduced after knockdown of ACSL4, but the inhibitory activity of curcumin against HepG2 was significantly attenuated after knockdown of ACSL4 (Fig. 4C).