The results showed that there was a significant difference in the TMB index of AML between the high and low CFD expression groups (Figure 4A), meanwhile, in the low-expression group of CFD, there were more mutations in Runt-related transcription factor 1 (RUNX1), indicated that CFD may be associated with the mutation of RUNX1 (Figure 4B). The gene discussed is RUNX1; the disease is acute myeloid leukemia.