The risk of osteoporosis was significantly associated with age ≥ 55 years old (OR: 5.066; 95% CI: 4.074-6.4, p<0.0001), female gender (OR: 1.75; 95% CI: 1.497-2.045, p<0.0001) and MTHFR rs1801133 GA genotype (OR: 1.17; 95% CI: 1.018-1.346, p=0.0275) respectively. The gene discussed is MTHFR; the disease is osteoporosis.