In addition, we also conducted nude mouse tumorigenesis experiments and found that miR-424-5p inhibitor can significantly promote tumor growth, and the expression level of CHEK1 is significantly increased, which indicate that miR-424-5p, as a tumor suppressor gene for HCC, plays a regulatory role by negatively regulating the expression of CHEK1. This evidence concerns the gene CHEK1 and hepatocellular carcinoma.