NCOA4 and malignant colon neoplasm: This hypothesis was tested utilizing a CRISPR/Cas9 NCOA4 knockout model in HCT116 and SW480 colon cancer cell lines compared to a wild-type NCOA4 model and examined growth under iron-deficient and iron-replete conditions, and the authors concluded that ferritinophagy is not required for basal cellular growth in CRC-derived cells.135