Knockdown of Sestrin2 in the lung cancer cell line A549 suppressed the cancer stemness markers (OCT, SOX2, and Nanog), drug resistance markers, migration markers (Snail, ZEB1, and vimentin), and transcription factors (Nrf2 and HO-1) which indicates that the expression of Sestrin2 upregulated the progression of lung cancer 146. The gene discussed is NFE2L2; the disease is lung cancer.