To validate the impact of the NDR1-FBXO11-β-catenin signaling axis on prostate cancer metastasis at the organism level, we constructed PC3 cell lines with NDR1 overexpressed, FBXO11 overexpressed, NDR1 overexpressed with FBXO11 knocked out, and FBXO11 phosphorylation site inactivation caused by an introduced mutation. This evidence concerns the gene FBXO11 and Familial prostate cancer.