NRAS and colorectal carcinoma: To further validate of the association between the gene expression of TRPS1 and EMT in CRC patients, we analyzed 594 colorectal samples from the TCGA dataset, and found that TRPS1 expression showed a weak correlation with KRAS, NRAS, HRAS and BRAF (Spearman=0.24, 0.13, -0.24, 0.25, all p-values < 1e−9, Supplementary Fig. 17a–d), but discovered a significant direct relationship with ZEB1 (Spearman=0.76, p-values = 3.27e−114, Supplementary Fig. 17e) and with ZEB2 (Spearman=0.79, p-values = 3.32e−129, Supplementary Fig. 17f).