In the first step, SUM-159 and MDA-MB-231 triple-negative breast carcinoma cells were electroporated with the most efficient gRNA complexed with Cas9 protein, and a donor fragment containing the DFN-cassette with left and right homology regions corresponding to the IRS2 genomic sequence (Fig. 1A). This evidence concerns the gene IRS2 and triple-negative breast carcinoma.