Out of the 32 genes, ten genes (MIXL1, INSM2, HIST1H3F, INA, PYY, MIR129-2, HOXA11, PITX3, CELF6 and MCIDAS) were not expressed in either control liver or HCC tissue samples and five genes (EFNB2, MTHFD2, OVOL1, HIST3H2A and CHST2) showed no differences in expression between HCC and non-tumoral samples (Fig. 4A). This evidence concerns the gene MIXL1 and hepatocellular carcinoma.