ATG4B and amyotrophic lateral sclerosis: Using dPCR, we detected higher levels of ATG4B cryptic exon PSI in an ALS frontal cortex sample (1.97%) than in a control one (0.05%) (Fig. 2a).However, TARDBP mRNA expression was not altered (Fig. s2), suggesting that the levels of cryptic exon inclusion do not depend only on TDP-43 expression level but also from other factors (e.g.nuclear localization).