Naive CD4+ T cells from Sf3b1K700Efl/+/CD4Cre mice were transduced with retrovirus expressing green fluorescent protein (GFP; +EV) along or together with Anapc13 (+Anapc13) and differentiated into Tregs, followed by adoptive transfer of Treg differentiated GFP+ cells to Rag1−/− mice to inhibit colitis induced by adoptively transferred naive CD4+ T cells (fig. Here, RAG1 is linked to colitis.