Loss of expression of either Kindlin-2, TβRI or ITGB1 resulted in the inability of the MDA-MB-231-KO cells (Fig. 3A, B) or the 4T1 KO cells (Fig. 3C, D) to effectively close the scratch wound after 24 h, supporting the role of Kindlin-2, TβRI and β1-Integrin in cancer cell migration. This evidence concerns the gene ITGB1 and cancer.