These results provide direct evidence that MYC and NDME genomic loci colocalize in intranuclear N1ICD transcriptional condensates, and that their colocalization depends upon formation of an intact Notch1 transcriptional activation complex, suggesting a role for N1ICD condensates in mediating MYC enhancer/promoter interactions. This hypothesis is further supported by our observations that endogenous Notch activity is necessary for MYC expression and that OptoNotch activation increases MYC expression as quantified by qPCR and RNA in situ hybridization in both T-ALL and HEK293 cells. Here, NOTCH1 is linked to acute lymphoblastic leukemia.