With regard to the total range of volumes and number of condensates per cell we observe that OptoNotch works within the range of endogenous Notch1 condensates formed from DeltaMAX treated and untreated T-ALL cells, compared to total nuclear intensity and individual intensity were there are several data points outside of the working range of both DeltaMAX treated or untreated T-ALL cells. Here, NOTCH1 is linked to acute lymphoblastic leukemia.