Lastly, to demonstrate the ability of OptoNotch to rescue MYC expression, and thus provide supporting evidence of MYC super-enhancer looping through functional association between the MYC and NDME loci, we next performed qPCR on MYC expression in both T-ALL cells, which exhibit a Notch dependency for MYC expression, and in HEK293 cells7–9,51,52,66. This evidence concerns the gene MYC and acute lymphoblastic leukemia.