This activity is mediated by the enhancement of FOXO3 expression by metformin, which subsequently increases NLRP3 transcription and facilitates pyroptosis.335 Another camptothecin anticancer drug, FL118, can also inhibit the progression and metastasis of colorectal cancer by inducing NLRP3-ASC-Caspase-1 mediated pyroptosis.336 A significant correlation was noted between the expression of GSDMD and the presence of NEK7. This evidence concerns the gene CASP1 and colorectal cancer.