PADI4 and cancer: The results of experiments with PAD4 inhibitor-treated cell lines or with neutrophils from mice with PAD4 deficiency are difficult to interpret because of low NET yields.135 For example, PAD4 inhibition prevents NET formation activated by nicotine rather than cholesterol crystals.24,155 However, studies with a variety of NET markers have shown that inhibition of PAD4 suppresses NET release in murine models of sepsis and cancer.