To further confirm the role of Ptges in tumor growth and to exclude a possible role of Cas9 antigenicity causing suppressed tumor growth, we knocked down (KD) Ptges in the same 6419c5 TL PDAC cell line through doxycycline-inducible (DOX-inducible) shRNA (TetR Ptges KD). The gene discussed is PTGES; the disease is neoplasm.