Immunohistochemical staining confirmed that there was no lymph node metastasis (0/24); immunohistochemistry staining results: cancer cells CK8/18+,Syn-, CgA-, p53 (+), C-erbB-2 (0), Ki67 (40%+); MLH1+, PMS-2+, MSH-2+, MSH-6+. The gene discussed is TP53; the disease is cancer.