The 30 TSS positive control gRNAs were selected from a previously reported hCRISPRa-v2 library16, while the 313 candidate promoter-targeting gRNAs were designed to 50 annotated TSSs of 9 high-confidence haploinsufficient risk genes associated with ASD and NDD (BCL11A, TCF4, ANK2, CHD8, TBR1, SCN2A, SYNGAP1, FOXP1, and SHANK3)17. Here, FOXP1 is linked to Neurodevelopmental delay.