Our pathway enrichment analyses highlighted RUNX1 targets involved in leukemogenesis, including multiple zinc finger proteins (of which ZNF384 is known to be important in MPAL), as well as ALDH57, ARHGAP58, ETV59,60, FANC61, GATA62, HOX63, HSP64, LMO65, METTL66, and TRIM67 family genes. This evidence concerns the gene TRIM67 and mixed phenotype acute leukemia.