MAPT and supranuclear palsy, progressive, 1: Western blot analysis to compare Tau zebrafish with human PSP midbrain lysates, extracted with either RIPA (1% Triton, 0.1% SDS) or DIGE (7 M urea), showed co-migration of multiple Tau-immunoreactive bands, further demonstrating similarity between the model and PSP (Fig. 4a).