Theoretical analysis of the NO content results and the effect of the CaMBD amino acid sequence in nNOS on its interaction with CaM provides novel insights into potential pathological pathways for inhibiting nNOS in cognitive decline and myopathy (Kelley et al., 2009; Kelley et al., 2011; Ito et al., 2013; Pavesi et al., 2013; Baldelli et al., 2014; James et al., 2015). This evidence concerns the gene NOS1 and myopathy.