Figures 7E, F shows that knockdown of EIF5A alone did not yield statistically significant changes in tumor volume. However, by day 14, the mean tumor volume in the shEIF5A+IR group exhibited a significant reduction compared to the other experimental groups (both P<0.01, Figures 7E, F). Immunofluorescence analysis indicated an increase in CD8+ T cells and M1 macrophages, and a decrease in M2 macrophages and Tregs in the shEIF5A+IR group compared to the NC, shEIF5A, and IR groups (Figure 7G). This evidence concerns the gene EIF5A and neoplasm.