Among them subclusters 1, 2, 3 were increased in Ischemic stroke compared to the sham group (Figure 7I), combined with Figures 7G, H the three subclusters increased subclusters 1 and 3 were probably CD8(TRLs) cells, where the IS-RSGs gene set score in the subclusters was not seen to be significantly reduced (Supplementary Figure 3D). This evidence concerns the gene CD8A and ischemic stroke.