MCM4 and melanoma: To further demonstrate the effect of MCM4 knockdown on erastin-induced cell death in vivo, B16F0 melanoma cells were subcutaneously inoculated into C57BL/6 mice with sh-NC (B16F0 melanoma cells stably expressing control shRNA) or sh-MCM4 (B16F0 melanoma cells stably expressing MCM4-targeting shRNA).