Markers for epithelium (KRT14), fibroblasts (FAP), macrophages (CD68), vasculature (CD31), T cells (CD3), and neutrophils (MPO) were clearly visualized in the CODEX fluorescent images (Fig. 3b); tumor state specific markers (TROP2 and CHI3L1) further allowed the spatial lineation of BST and BIT subregions of tumors respectively (Fig. 3c). Here, CHI3L1 is linked to neoplasm.