Consistent with our findings in the HeLa reporter cell lines and HEK293 cells, both C9orf72 RNA and protein levels remained unaffected in the Cas13d-S24 and Cas13d-S30 groups compared with the non-targeting Cas13d-NT30 group in the 4 normal iPSC and iMN cell lines (Supplemental Figure 2, B–G), indicating a highly selective targeting by the CRISPR/Cas13d system against the expanded alleles in C9-ALS patient cells. Here, C9 is linked to amyotrophic lateral sclerosis.