Specifically, the C9-ALS iPSC line 1 and iMN line 1 carried a 5× GGGGCC non-expanded allele, making them targetable by S24- and S30-guided Cas13d, as demonstrated by the in vitro cleavage assay (Figure 4C), potentially explaining the reduction of the repeat-containing transcripts C9-V1 and C9-V3 in both cell lines. This evidence concerns the gene C9 and amyotrophic lateral sclerosis.