MYC and hepatocellular carcinoma: Using a spontaneous HCC model generated by hydrodynamic transfection of Myc, gp53/Cas9, and sleeping beauty transposase for stable genomic integration in C57BL/6J mice as previously described (29), we observed that murine livers with HCC have elevated ammonia concentrations in tumors when compared with normal control livers (Figure 7A).