These results demonstrate, for the first time, that NA Vglut2-based glutamatergic signaling is dispensable for respiratory control, which challenges the prevalent perspective on the role of C1 and other Vglut2-expressing NA neurons in respiratory homeostasis and suggests that glutamate may not be a critical target to understand NA neuron dysfunction in respiratory diseases. The gene discussed is SLC17A6; the disease is respiratory system disorder.