We found that ovarian cancer cells treated with carboplatin in combination with CCL2/MCP-1 for 48 hours had increased SOX2, OCT4, and NANOG gene expression (Supplementary Fig. S6C and S6D) and greater spheroid formation ability relative to carboplatin or CCL2/MCP-1 treatment alone (Fig. 7D). This evidence concerns the gene NANOG and ovarian cancer.