Therefore, to investigate if the V. cholerae DarTG system restricts ICP1 genome replication in its native context, we calculated ICP1 genome replication efficiency after a single round of infection of PDE+V. cholerae, the permissive PDE- strain or a newly engineered PDE+ strain expressing the catalytically dead mutant of DarT. This evidence concerns the gene ALDH7A1 and infection.