NKAIN1 and breast cancer: NKAIN1 is a mammalian protein belonging to a family of proteins that shares similarities with Drosophila Nkain and interacts with the beta subunit of Na,K-ATPase(Na+/K + pump) and has been implicated in ion transport and regulation of cell volume.[45] Yusha Liu et al identified NKAIN1 as one of the signature genes in BC patients and showed a positive correlated with Macrophage.[46] In our study, we found NKAIN1 was positively associated with Macrophages M2, while negatively associated with Macrophages M1.