To dissect the molecular mechanism by which PTBP1 promotes radioresistance in PCa, we first investigated whether PTBP1 modulates the AS of MESI2, PKM, and AXL, all of which have been reported to regulate tumor progression.[19] Unexpectedly, the AS of these genes was not regulated by PTBP1, suggesting that other alternative genes are involved in PTBP1‐mediated radioresistance in PCa (Figure S3a, Supporting Information). This evidence concerns the gene PKM and neoplasm.