found that PTBP1 drives liver cancer tumorigenesis by promoting cancer metabolic switch.[25] Our previous data indicated that PTBP1 promotes proliferation and metastasis of bladder cancer.[19] Differently from previous works, our data in this study first demonstrated that PTBP1 overexpression could efficiently elicit radiotherapy resistance of PCa cells by regulating aberrant exon skipping of DNMT3B, which further broadened knowledge about PTBP1 regulatory role in tumor progression. Here, DNMT3B is linked to urinary bladder cancer.