Likewise, incubation of IEC‐6 cells with conditioned medium derived from silenced‐NLRP3 EGCs counteracted the reduction of tight junction expression along with the increase of trans‐epithelial permeability (Figure 7A,C–G), thus further corroborating the contribution of glial NLRP3 inflammasome in obesity‐related intestinal barrier impairment. This evidence concerns the gene NLRP3 and obesity disorder.