In order to verify the existence of a link among NLRP3 inflammasome activation, enteric reactive gliosis and intestinal mucosal barrier alteration associated with obesity, we investigated the mucus layer composition, the expression of tight junction proteins (proteins involved in the maintenance of mucosal barrier integrity) and ZO‐1 localization in colonic tissues from SD‐ and HFD‐WT and NLRP3−/− mice. The gene discussed is NLRP3; the disease is Obesity.