In order to investigate the potential relationship between NLRP3 inflammasome activation, enteric gliosis, and alterations of intestinal mucosal barrier in the setting of obesity, we performed in vitro experiments on coculture of EGCs and intestinal epithelial cells (IEC‐6 cells) treated with palmitate (PA) and lipopolysaccharide (LPS), to mimic the in vivo features of HFD exposure. Here, NLRP3 is linked to obesity disorder.