NLRP3 and obesity due to melanocortin 4 receptor deficiency: In order to verify the existence of a link among NLRP3 inflammasome activation, enteric reactive gliosis and intestinal mucosal barrier alteration associated with obesity, we investigated the mucus layer composition, the expression of tight junction proteins (proteins involved in the maintenance of mucosal barrier integrity) and ZO‐1 localization in colonic tissues from SD‐ and HFD‐WT and NLRP3−/− mice.