To further assess in detail the efficacy of alginate lyases in the cystic fibrosis lung milieu, we assayed the alginate lyases, CaAly, CaFLDAly, SA1-III, SA1-IV, FspAlyFRB, PspCy24AlyP1, PspAlgL, and VspAlyVI, in the presence of single divalent metal ions, magnesium (Mg2+), manganese (Mn2+), calcium (Ca2+), iron (Fe2+), nickel (Ni2+), copper (Cu2+), and zinc (Zn2+). This evidence concerns the gene STAG1 and cystic fibrosis.