Furthermore, qPCR assays revealed that HA-PHB2S91A transfection blunted the positive effect of Pgam5 deletion on mitochondrial biogenesis by reinstating transcriptional downregulation of Pgc1α and Nrf2 in hyperglycemia-exposed cells (Fig. 7I and J). The gene discussed is PPARGC1A; the disease is Hyperglycemia.