Based on these bioinformatics analyses, we found that PD-L2 expression was high in two metastatic T24L and 253J-BV cells and 253J and UMUC13 had low PD-L2 expression, and parental T24 had intermediate PD-L2 expression, however, PD-L1 expression is not detected in most of bladder cancer cell lines except parental T24 cell (Fig. 4D). The gene discussed is PDCD1LG2; the disease is urinary bladder cancer.