Knowing the PI3K/Akt/β-catenin pathway as an essential signaling regulates migration, invasion, and metastasis of several cancer types cancer development [26–28], we transiently overexpressed wild-type β-catenin in both SPHK1 knockout subline T24LsgSPHK1#A and 253J-BVsgSPHK1#B to confirm whether β-catenin can restore PD-L2 expression. The gene discussed is SPHK1; the disease is cancer.