Analysis performed in the culture medium of primary cell lines we generated from patients with soft-tissue sarcoma histologies other than EHE, such as pleomorphic liposarcoma, dedifferentiated liposarcoma, or angiosarcoma, as well as of a normal endothelial cell line (HUVEC) showed a lack or a negligible level of released GDF-15 (Fig. 2B). This evidence concerns the gene GDF15 and angiosarcoma.