MC4R and obesity due to melanocortin 4 receptor deficiency: In the case–control study of 578 Pakistani adults, the researchers further investigated the effects of interaction between the 5 SNPs, MC4R rs17782313, FTO rs1421085, TMEM18 rs7561317, NEGR1 rs2815752, and BDNF rs6265 and several unhealthy lifestyle factors (including random eating patterns, a moderate-to-high tendency toward fat-dense food, inadequate sleep [<7 hours/day], irregular sleep–wake cycle, and shift work) on obesity risk.64