However, in myoblasts, all the detectable TDP-43 was located in the nucleus in patient- and control-derived myoblasts, including in cells carrying a TARDBP mutation (fALS_1 patient) (Supplementary Fig. 3a), and whilst some FUS was found in cytoplasmic granules, these were of equal abundance in CTRL and ALS myoblasts (Supplementary Fig. 3b). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.