FUS and amyotrophic lateral sclerosis: However, in myoblasts, all the detectable TDP-43 was located in the nucleus in patient- and control-derived myoblasts, including in cells carrying a TARDBP mutation (fALS_1 patient) (Supplementary Fig. 3a), and whilst some FUS was found in cytoplasmic granules, these were of equal abundance in CTRL and ALS myoblasts (Supplementary Fig. 3b).