It is an indirect fluorescent antibody test system (HEp-2000® fluorescent IgG ANA-Ro IVD kit by Immunoconcepts, USA) for the semi-quantitative detection of ANA IgG in serum among all the dengue suspected patient by manual fluorescent microscopy (Ayrus Epi microscope with LED epifluorescence technology by Medsource Ozone Biomedicals Pvt. The gene discussed is BTG3; the disease is dengue disease.