Western blot demonstrated that HF mice subjected to HIIT had higher hind-limb muscle Metrnl, compared with non-HIIT (Fig. 2A, B), which was further supported by immunofluorescent staining, where Metrnl was localized within skeletal muscle fibers, and had higher expression levels among HF + HIIT versus HF alone (Fig. 2C). Here, METRNL is linked to hydrops fetalis.