Additionally, the 99mTc-labeled Nb C7 was used to visualize the spatiotemporal expression of mPD-L1 in mRNA-vaccinated B16 tumor-bearing mice, showing significant PD-L1-upregulation as early as one day post-vaccination, suggesting a potential synergy between cancer vaccination and PD-L1-blockade [30]. This evidence concerns the gene CD274 and cancer.