In the current research, we revealed that lncRNA HISLA from TAM-exo could be taken up by bladder cancer cells, and silencing of HILSA in TAM-exo alleviated the metastasis, invasion, and EMT marker expression of bladder cancer cells, which indicated that exosomal lncRNA HISLA derived from TAMs promoted EMT potential of bladder cancer cell. This evidence concerns the gene HISLA and urinary bladder cancer.