The relative mRNA expression was lower in PLX5622 CSOM (0.25 ± 0.14) than in control CSOM (1.24 ± 0.29) for NLRP3 (p = 0.01), in PLX5622 CSOM (0.54 ± 0.16) than in control CSOM (1.04 ± 0.13) for Pycard (p = 0.033), in PLX5622 CSOM (0.27 ± 0.2) than in control CSOM (1.17 ± 0.26) for Caspase-1 (p = 0.017), in PLX5622 CSOM (0.42 ± 0.16) than in control CSOM (1.12 ± 0.26) for IL-1β (p = 0.04), and in PLX5622 CSOM (0.39 ± 0.17) than in control CSOM (1.1 ± 0.17) for IL-18 (p = 0.012). Here, NLRP3 is linked to chronic purulent otitis media.