Monocytes are isolated and differentiated into immature DCsusing GM‐CSF and IL‐4DCs are loaded with tumour‐associated antigens such as peptides, nucleic acids, or tumour lysateAntigen‐loaded DCs mature using a cytokine mixture, including TNF‐α, IL‐1β, IL‐6 and prostaglandin E2138DCs can be electroporated with mRNA encoding CD70, CD40 ligand, constitutively active TLR4 (TriMix), and tumour‐associated antigens to enhance antigen presentation and immune stimulation139. The gene discussed is CSF2; the disease is neoplasm.