CKD rats treated with standard carotenoids (STDCar) or gac fruit extract combined with probiotics (GacPro) displayed normalized ZO-1 expression, while isolated gac fruit extract (GacCar) or standard probiotics with carotenoid extract (STDPro) could partially rescue the expression of ZO-1 (Figure 3B). The gene discussed is TJP1; the disease is chronic kidney disease.