In this study, we developed a 3D structure-based pharmacophore model [5,6] specifically built for accelerating the identification of BRD4 inhibitors, based on the chemical information from the crystal structure of this bromodomain-containing protein with (+)-JQ1 [7], which is a well-known potent BET inhibitor featuring potent anti-proliferative effects in multiple cancer models by binding BRD4 and preventing its interaction with chromatin. The gene discussed is BRD4; the disease is cancer.