Our results indicate the potential significance of TLR3 and TLR8 (endosomal receptors) and TLR4 (membrane receptor) in responding to HPV infection and its progression to SILs and ICC, highlighting the importance of HPV polyinfection in relation to TLR4 and TLR8. The gene discussed is TLR8; the disease is intrahepatic cholangiocarcinoma.