The present results suggest that C3 deficiency-induced constipation may be associated with 1237 upregulated genes including PNLIP, CEL, CPB1, CTRL, PNLIPRP1, and REG1 as well as 1292 downregulated genes including Eif2s3y, UTY, KDM5D, IGKV6-32, Olfr870, and DDX3Y. Here, PNLIPRP1 is linked to constipation disorder.